Topic 11.1 Antibody Production and Vaccination
In the Antibody Production and Vaccination unit will will learn how immunity is based on the fact that an animal's body can recognize cells and proteins that are of "it-self" and distinguish those that are foreign or 'nonself'. We will learn how foreign material can be identified and destroyed.
This unit will last 4 school days
This unit will last 4 school days
Essential idea:
- Immunity is based on recognition of self and destruction of foreign material.
Nature of science:
Edward Jenner was a scientist who infected a small child with cowpox. After the boy recovered he then affected the child with the more virulent and possibly fatal smallpox, as he believed the child would be immune because of the original cowpox infection. He did this on a young child well below the age of consent
Jenner’s Story
Ethics in Medicine:
- Consider ethical implications of research—Jenner tested his vaccine for smallpox on a child. (4.5)
Edward Jenner was a scientist who infected a small child with cowpox. After the boy recovered he then affected the child with the more virulent and possibly fatal smallpox, as he believed the child would be immune because of the original cowpox infection. He did this on a young child well below the age of consent
Jenner’s Story
Ethics in Medicine:
Understandings:
11.1 U 1 Every organism has unique molecules on the surface of its cells.
All organisms have unique molecules or markers on the outer surface of the plasma membrane of their cells. These highly variable molecules are generally glycoproteins and they identify a cell as being “self” or “non-self”. These markers are called major histocompatibility complexes (MHC). These MHC proteins are genetically determined and are unique to that individual
image from Learn Genetics
11.1 U 2 Pathogens can be species-specific although others can cross species barriers.
Invading organisms such as a virus or bacterium that enters the body and causes a disease are known as pathogens. Pathogens are generally species specific, for example, humans are the only known organisms susceptible to pathogens such as polio, syphilis, measles and gonorrhea but are resistant to many pathogens that infect other organisms. However, there are pathogens that can cross this species barrier and infect a range of hosts, such as the Rabies virus, bird flu and the Bubonic plague
Diseases from other animals that can infect or be transmitted to humans is called Zoonosis. The passing of diseases from different species is a growing global health concern
Diseases from other animals that can infect or be transmitted to humans is called Zoonosis. The passing of diseases from different species is a growing global health concern
image from PLOS
11.1 U 3 B lymphocytes are activated by T lymphocytes in mammals.
When a pathogen enters the blood, the specific antigen on the surface of the membrane is identified. Specific phagocytes known as macrophages recognize a pathogen as a foreign entity because of the antigens on the surface.. The macrophage engulfs and partially destroys the pathogen. The macrophage takes the antigens from the destroyed pathogen and displays them on the surface of the cell bound to a membrane protein called a MHC protein (called antigen presentation).
Specific T-lymphocytes receptors recognize and bind to the antigen presented by the macrophage, thus activating the T-lymphocyte. The activated T-cell binds to a B-lymphocyte specific to the antigen; activating the B-cell through the binding and the release of a signaling protein
Specific T-lymphocytes receptors recognize and bind to the antigen presented by the macrophage, thus activating the T-lymphocyte. The activated T-cell binds to a B-lymphocyte specific to the antigen; activating the B-cell through the binding and the release of a signaling protein
11.1 U 4 Activated B cells multiply to form clones of plasma cells and memory cells.
The active B-cells begin to clone themselves (by mitosis) producing cloned plasma B cells that produce antibodies and memory cells. The generation of large numbers of plasma cells that produce one specific antibody type is known as clonal selection. Memory cells remain in the blood in case a second infection occurs to provide long term protection and a quick response to the new infection. The plasma cells created produce and release mass amounts of antibodies into the bloodstream. These antibodies surround and bind to the antigens on the foreign pathogens. Through a variety of different methods the pathogens are destroyed by the antibodies and other white blood cells.
image from BC Open Textbooks
11.1 U 5 Plasma cells secrete antibodies.
Plasma cells are specialized B lymphocytes (called B cells as they develop in the bone marrow) that secrete a large amount of antibodies during a selective immune response. Since they are a cell that produces and secretes a large number of antibodies (proteins), they contain an extensive amount of rER, ribosomes and mitochondria (for energy).
image from Action International
11.1 U 6 Antibodies aid the destruction of pathogens.
- Agglutination – Antibodies cause the sticking together of pathogens by attaching to the antigens on the surface. These clumped masses of pathogens are then easily ingested and destroyed by phagocytes. The large agglutinated mass can be filtered by the lymphatic system and then phagocytised.
- Opsonisation – Antibodies make pathogens recognizable by binding to them and linking them to phagocytes.
- Neutralisation of toxins – Antibodies bind to toxins produced by pathogens in the blood plasma preventing them from affecting susceptible cells.
- Complement activation – The complement system is a collection of proteins which ultimately lead to the perforation of the membranes of pathogens. Antibodies bound to the surface of a pathogen activate a complement cascade which leads to the formation of a “membrane attack complex” that forms a pore in the membrane of the pathogen allowing water and ions to enter into the cell, causing the cell to lyse.
- Neutralisation of viruses and bacteria – Antibodies can bind to the surface of viruses, preventing them from entering host cells.
11.1 U 7 White cells release histamine in response to allergens.
Histamine is an important protein involved in many allergic reactions. Allergies are caused by an immune response to a normally innocuous substance (i.e. pollen, dust) that comes in contact with lymphocytes specific for that substance, or antigen. In many cases, the lymphocyte triggered to respond is a mast cell. For this response to occur, a free-floating IgE (an immunoglobulin associated with allergic response) molecule specific to the antigen must first be attached to cell surface receptors on mast cells. Antigen binding to the mast cell-attached IgE then triggers the mast cell to respond. This response often includes the release of histamine. It causes contraction of smooth muscle and dilation of capillaries.
11.1 U 8 Histamines cause allergic symptoms.
Histamine causes many of the symptoms of allergies, such as a runny nose or sneezing. When a person is allergic to a particular substance, such as a food or dust, the immune system mistakenly believes that this usually harmless substance is actually harmful to the body. In an attempt to protect the body, the immune system starts a chain reaction that prompts some of the body's cells to release histamine and other chemicals into the bloodstream. The histamine then acts on a person's eyes, nose, throat, lungs, skin, or gastrointestinal tract, causing allergy symptoms.
NOTE: this is why people with allergies take antihistamines!
NOTE: this is why people with allergies take antihistamines!
11.1 U 9 Immunity depends upon the persistence of memory cells.
Immunity develops when the immune system is challenged by a specific antigen and produces antibodies and memory cells in response. Memory cells ensure that the second time an antigen is encountered, the body is ready to respond rapidly by producing more antibodies at a faster rate.
11.1. U 10 Vaccines contain antigens that trigger immunity but do not cause the disease.
Vaccines are introduced to the body usually through an injection but can be administered through orally or through a nasal spray. Vaccines contain a live attenuated (weakened) or killed version of the pathogen, its toxins or one of its surface antigens. Vaccines help to stimulate a primary immune response.
If the body encounters the actually pathogen, it will be destroyed right away by the antibodies during a secondary immune response. Vaccines has made great contributions towards public health through the prevention of many deadly or dangerous diseases such as tuberculosis, measles and smallpox
If the body encounters the actually pathogen, it will be destroyed right away by the antibodies during a secondary immune response. Vaccines has made great contributions towards public health through the prevention of many deadly or dangerous diseases such as tuberculosis, measles and smallpox
11.1 U 11 Fusion of a tumour cell with an antibody-producing plasma cell creates a hybridoma cell.
Monoclonal antibodies are highly specific, purified antibodies that are produced by a clone of cells, derived from a single cell. They recognise only one antigen. To produce the clone of cells that will manufacture a monoclonal antibody, the antigen recognised by the antibody is injected into a mouse, or other mammal. In response to this challenge, the mouse's immune system makes plasma B cells that are capable of producing the desired antibody. Plasma cells are removed from the spleen of the mouse. They will be of many different types with only some producing the desired antibody.
The B cells are fused with cancer cells called myeloma cells. The cells formed by fusion of plasma B cells and myeloma cells are called hybridoma cells.
The B cells are fused with cancer cells called myeloma cells. The cells formed by fusion of plasma B cells and myeloma cells are called hybridoma cells.
11.1. U 12 Monoclonal antibodies are produced by hybridoma cells.
- Monoclonal antibodies are identical antibodies produced by clones of a single parent immune cell that are specific to one type of antigen.
- A laboratory animal such as a mouse is injected with a specific antigen thatcorresponds with the needed antibodies.
- After the animal goes through a primary immune response, a plasma B-cell cell that produces the required antibody is removed from the spleen.
- Myeloma (cancer) cells are cultured in a petri dish.
- These dividing myeloma cells are mixed together with the plasma B-cells and are treated to promote a fusion between the two cells, forming a cell called a hybridoma.
- The successful hybridomas have characteristics of both cells; produce antibodies and divide rapidly for a long time.
- These hybridoma cells are cultured and allowed to divide, producing many clone cellsthat are able to produce large amounts of antibodies.
- Monoclonal antibodies can be extracted and used for many different applications.
image from Research Gate
Applications
11.1 A 1 Smallpox was the first infectious disease of humans to have been eradicated by vaccination
In 1959 a global initiative was undertaken by the WHO in order to eradicate smallpox. The effort had mixed results until a well-funded Smallpox Eradication Unit was formed in 1967. The last known case of smallpox was recorded in Somalia in 1977.
It was successful because of the following reasons
It was successful because of the following reasons
- Patients were easily identified by obvious clinical features
- Transmission was through direct contact only
- There were no animal vectors or reservoirs where the disease could remain and reemerge
- Contacts of the patients identified were quickly identified and vaccinated
- Immunity was long term so reinfection was unlikely
- The infection period was short-lived (3 to 4 weeks)
- The virus was stable and didn’t mutate
- There was international cooperation organized by the WHO
11.1 A 2 Monoclonal antibodies to HCG are used in pregnancy test kits.
Use in diagnosis of pregnancy
Use in treatment of rabies
**Other examples are treatment of cancer cells and detection of HIV**
- Human chorionic gonadotrophin (HCG) is produced by an embryo in early pregnancy.
- Monoclonal antibodies can be produced by injecting a lab animal with HCG, as it recognizes this as antigen.
- HCG Antibodies are combined with color-changing enzymes.
- When the mixture is introduced into a blood sample of a woman that is pregnant, theantibodies will bind to the HCG in the blood, causing a change in color.
- If the woman is not pregnant, no HCG will be present in her blood, and therefore there will be no color change.
Use in treatment of rabies
- Monoclonal antibodies are produced using the method described in 11.1.5.
- The antibodies are injected directly into the person after a possible rabies infection.
- The antibodies will control and fight the infection, giving time for the body to produce its own antibodies.
- If not treated with antibodies after a rabies infection, death can result.
**Other examples are treatment of cancer cells and detection of HIV**
image from Point of Care Devices
11.1 A 3 Antigens on the surface of red blood cells stimulate antibody production in a person with a different blood group
- Blood groups such as A, B, AB and O are identified by cell surface antigens
- Rhesus (Rh) is another antigen that can be present on the surface of the blood cells, being either Rh positive (has antigen) or Rh negative (doesn’t have antigen)
- A blood transfusion given to an individual with the wrong blood type can stimulate an immune response called agglutination (clumping or clotting of the blood cells)
- This is followed by the destruction of the RBC (hemolysis)
- For example, someone with blood type A (antigen A on the surface) contains anti-B antibodies in their plasma. If they get a transfusion with blood type B, their immune system will attack and destroy the foreign blood cells with the B-antigen on the surface
- People with blood type O just have the basic antigen sequence that all blood cells have and are therefore not attacked by A or B antibodies; therefore, blood type O is known as the universal donor (O negative has no Rhesus factor)
image from Alyvea.com
Skills:
11.1 S 1 Analysis of epidemiological data related to vaccination programmes.
Epidemiological Studies on Vaccinations
Key Terms
|
antibody
memory cells primary immunity zoonosis hybridoma cells hemolysis epidemiology myeloma cells |
immunity
B-lymphocytes secondary immunity histamine ABO blood antigens smallpox Jenner |
antigen
T lymphocytes vaccination anti-histamine red blood cells eradicate opsonization |
challenge and response
helper T cells pathogen allergic symptoms monoclonal antibody pregnancy blood transfusion |
macrophage
plasma B cells crossing species tumor cell agglutination HCG toxin |
PowerPoint and Notes on Topic 11.1 by Chris Payne
Correct use of terminology is a key skill in Biology. It is essential to use key terms correctly when communicating your understanding, particularly in assessments. Use the quizlet flashcards or other tools such as learn, scatter, space race, speller and test to help you master the vocabulary.
Useful Links
In the News
International-mindedness:
- The World Health Organization initiated the campaign for the global eradication of smallpox in 1967. The campaign was deemed a success in 1977, only 10 years later.
Video Clips
