topic 1.4: membrane transport
In the Cell Membrane unit we will learn that the cell membrane is one of the great multi-taskers of biology. It provides structure for the cell, protects cytosolic contents from the environment, and allows cells to act as specialized units. We will also learn that the membrane is the cell’s interface with the rest of the world - it’s gatekeeper. This phospholipid bilayer determines what molecules can move into or out of the cell, and so is in large part responsible for maintaining the delicate homeostasis of each cell.
The unit is planned to take 3 school days
The unit is planned to take 3 school days
Essential Ideas:
- Membranes control the composition of cells by active and passive transport.
Nature of Science
- Experimental design—accurate quantitative measurement in osmosis experiments are essential. (3.1) (covered by the practical and processing of data)
- Define quantitative and qualitative.
- Determine measurement uncertainty of a measurement tool.
- Explain the need for repeated measurements (multiple trials) in experimental design.
- Explain the need to control variables in experimental design.
- Define quantitative and qualitative.
Understandings
1.4.U.1 Particles move across membranes by simple diffusion, facilitated diffusion, osmosis and active transport. (Oxford Biology Course Companion page 35).
- Describe simple diffusion.
- Explain two examples of simple diffusion of molecules into and out of cells.
- Outline factors that regulate the rate of diffusion.
- Describe facilitated diffusion.
- Describe one example of facilitated diffusion through a protein channel.
- Define osmosis.
- Predict the direction of water movement based upon differences in solute concentration.
- Compare active transport and passive transport.
- Explain one example of active transport of molecules into and out of cells through protein pumps.
Cellular membranes possess two key qualities:
Movement of materials across a biological membrane may occur either actively or passively
Diffusion is the passive movement (does not require energy) of particles from a region of high concentration to a region of low concentration.
Osmosis - the passive movement of water molecules, across a semi-permeable membrane, from a region of lower solute concentration to a region of higher solute concentration.
Simple diffusion - passive movement of particles from an area of high concentration to an area of low concentration (follows its concentration gradient). Simple diffusion across membranes occurs when substances other than water move across the phospholipid bilayer (between the phospholipids) or through protein channels.
Facilitated Diffusion - specific ions and other particles that cannot move through the phospholipid bilayer sometimes move across protein channels
Active transport - movement of substances across membranes using energy from ATP.
- They are semi-permeable (only certain materials may freely cross – large and charged substances are typically blocked)
- They are selective (membrane proteins may regulate the passage of material that cannot freely cross)
Movement of materials across a biological membrane may occur either actively or passively
Diffusion is the passive movement (does not require energy) of particles from a region of high concentration to a region of low concentration.
- Passive transport means there is no expenditure of energy (ATP).
- Passive transport requires the substance to move from an area of high concentration to low concentration.
Osmosis - the passive movement of water molecules, across a semi-permeable membrane, from a region of lower solute concentration to a region of higher solute concentration.
Simple diffusion - passive movement of particles from an area of high concentration to an area of low concentration (follows its concentration gradient). Simple diffusion across membranes occurs when substances other than water move across the phospholipid bilayer (between the phospholipids) or through protein channels.
- Substances that move across the membrane are usually small non-charged particles (i.e. Oxygen, Carbon Dioxide, and Nitrogen) or other lipids.
Facilitated Diffusion - specific ions and other particles that cannot move through the phospholipid bilayer sometimes move across protein channels
- During facilitated diffusion the membrane protein changes its shape to allow a specific substance to move across the membrane.
- Each protein channel structure allows only one specific molecule to pass through the channel. For example, magnesium ions pass through a channel protein specific to magnesium ions.
Active transport - movement of substances across membranes using energy from ATP.
- Active transport generally moves substances against their concentration gradient (low to high concentration).
- Many different protein pumps are used for active transport. Each pump only transports a particular substance; therefore cells can control what is absorbed and what is expelled.
- Pumps work in a specific direction; substances enter only on one side and exit through the other side.
- Substances enter the pump from the side with a lower concentration.
- Energy from ATP is used to change the conformational shape of the pump.
- The specific particle is released on the side with a higher concentration and the pump returns to its original shape.
1.4.U 2 The fluidity of membranes allows materials to be taken into cells by endocytosis or released by exocytosis. (Oxford Biology Course Companion page 34/35).
- Describe the fluid properties of the cell membrane and vesicles.
- Explain vesicle formation via endocytosis.
- Outline two examples of materials brought into the cell via endocytosis.
- Explain release of materials from cells via exocytosis.
- Outline two examples of materials released from a cell via exocytosis.
The membrane is principally held together by weak hydrophobic associations between the fatty acid tails of phospholipids. This weak association allows for membrane fluidity and flexibility, as the phospholipids can move around to some extent. This allows for the spontaneous breaking and reforming of the bilayer, allowing larger materials to enter or leave the cell without having to cross the membrane (this is an active process and requires ATP hydrolysis)
Endocytosis
- Plasma membrane is pinched as a result of the membrane changing shape.
- External material (i.e. Fluid droplets) are engulfed and enclosed by the membrane.
- A vesicle is formed that contains the enclosed particles or fluid droplets, now moves into the cytoplasm.
- The plasma membrane easily reattaches at the ends that were pinched because of the fluidity of the membrane.
- Vesicles that move through the cytoplasm are broken down and dissolve into the cytoplasm.
Exocytosis
- After a vesicle created by the rough ER enters the Golgi apparatus, it is again modified, and another vesicle is budded from the end of the Golgi apparatus, which moves towards the cell membrane.
- This vesicle migrates to the plasma membrane and fuses with the membrane, releasing the protein outside the cell through a process called exocytosis.
- The fluidity of the hydrophilic and hydrophobic properties of the phospholipids and the fluidity of the membrane allows the phospholipids from the vesicle to combine to the plasma membrane to form a new membrane that includes the phospholipids from the vesicle.
1.4.U3: Vesicles move materials within cells (Oxford Biology Course Companion page 34).
- List two reasons for vesicle movement.
- Describe how organelles of the endomembrane system function together to produce and secrete proteins (rough ER, smooth ER, Golgi and vesicles).
- Outline how phospholipids and membrane bound proteins are synthesized and transported to the cell membrane.
After proteins have been synthesized by ribosomes they are transported to the rough endoplasmic reticulum where they can be modified. Vesicles carrying the protein then bud off the rough endoplasmic reticulum and are transported to the Golgi apparatus to be further modified. After this the vesicles carrying the protein bud off the Golgi apparatus and carry the protein to the plasma membrane. Here the vesicles fuse with the membrane expelling their content (the modified proteins) outside the cell. The membrane then goes back to its original state. This is a process called exocytosis. Endocytosis is a similar process which involves the pulling of the plasma membrane inwards so that the pinching off of a vesicle from the plasma membrane occurs and then this vesicle can carry its content anywhere in the cell.
Application
1.4.A.1 Structure and function of sodium–potassium pumps for active transport and potassium channels for facilitated diffusion in axons.
- Describe the structure of the sodium-potassium pump.
- Describe the role of the sodium-potassium pump in maintaining neuronal resting potential.
- Outline the six steps of sodium-potassium pump action.
- Describe the structure of the potassium channel.
- Describe the mechanism of potassium movement through the potassium channel.
- Explain the specificity of the potassium channel.
- Describe the action of the “voltage gate” of the potassium channel.
The axons in nerve cells transmit electrical impulses by translocating ions to create a voltage difference across the membrane. In the resting phase, the sodium-potassium pump releases sodium ions from the nerve cell, while potassium ions are accumulated in. When the neuron fires, these ions swap locations through facilitated diffusion using the sodium and potassium channels
Sodium-Potassium Pump
An integral protein that exchanges 3 sodium ions out of cell with two potassium ions into the cell.
The process of ion exchange against the gradient is energy-dependent and involves a number of key steps:
Three sodium ions bind to intracellular sites on the sodium-potassium pump
Sodium-Potassium Pump
An integral protein that exchanges 3 sodium ions out of cell with two potassium ions into the cell.
The process of ion exchange against the gradient is energy-dependent and involves a number of key steps:
Three sodium ions bind to intracellular sites on the sodium-potassium pump
- A phosphate group is transferred to the pump via the hydrolysis of ATP
- The pump undergoes a conformational change, translocating sodium across the membrane
- The conformational change exposes two potassium binding sites on the extracellular surface of the pump
- The phosphate group is released which causes the pump to return to its original conformation
- This translocates the potassium across the membrane, completing the ion exchange
1.4.A.2 Tissues or organs to be used in medical procedures must be bathed in a solution with the same osmolarity as the cytoplasm to prevent osmosis (Oxford Biology Course Companion page 44).
- Explain what happens to cells when placed in solutions of the same osmolarity, higher osmolarity and lower osmolarity.
- Outline the use of normal saline in medical procedures.
Hypertonic solution – Is a solution with a higher osmolarity (higher solute concentration) then the other solution. If cells are placed into a hypertonic solution, water will leave the cell causing the cytoplasm’s volume to shrink and thereby forming indentations in the cell membrane.
Hypotonic solution – Is a solution with a lower osmolarity (lower solute concentration) then the other solution. If cells are placed in a hypotonic solution, the water will rush into the cell causing them to swell and possibly burst.
Both of the above solutions would damage cells, therefore isotonic solutions are used (same osmolarity as inside the cell)
Isotonic solution: A solution that has the same salt concentration as cells and blood.
Organs placed in hypotonic solution or hypertonic solution would damage cells, therefore isotonic solutions are used (same osmolarity as inside the cell)
In medical procedures, isotonic solutions are commonly used as
Hypotonic solution – Is a solution with a lower osmolarity (lower solute concentration) then the other solution. If cells are placed in a hypotonic solution, the water will rush into the cell causing them to swell and possibly burst.
Both of the above solutions would damage cells, therefore isotonic solutions are used (same osmolarity as inside the cell)
Isotonic solution: A solution that has the same salt concentration as cells and blood.
Organs placed in hypotonic solution or hypertonic solution would damage cells, therefore isotonic solutions are used (same osmolarity as inside the cell)
In medical procedures, isotonic solutions are commonly used as
- Intravenously infused fluids in hospitalized patients.
- Used to rinse wounds and skin abrasions
- Saline eye drops
- Packing donor organs for transport (frozen to slush)
- During skin grafts, used to keep damaged area moist (normal saline is approx. 300 mOsm)
Skill
1.4.S.1 Estimation of osmolarity in tissues by bathing samples in hypotonic and hypertonic solutions. (Oxford Biology Course Companion page 41).
(Practical 2) [Osmosis experiments are a useful opportunity to stress the need for accurate mass and volume measurements in scientific experiments.]
(Practical 2) [Osmosis experiments are a useful opportunity to stress the need for accurate mass and volume measurements in scientific experiments.]
- Define osmolarity, isotonic, hypotonic and hypertonic.
- Calculate the percentage change between measurement values.
- Calculate the mean value of a data set.
- Calculate the standard deviation value of a data set.
- State that the term standard deviation is used to summarize the spread of values around the mean, and that 68% of the values fall within one standard deviation of the mean.
- Explain how the standard deviation is useful for comparing the means and the spread of data between two or more samples.
- Determine the correct type of graph to represent experimental results.
- State that error bars are a graphical representation of the variability of data.
- Accurately graph mean and standard deviation of data sets.
- Determine osmolarity of a sample given changes in mass when placed in solutions of various tonicities.
Osmolarity is a measure of solute concentration, as defined by the number of osmoles of a solute per litre of solution (osmol/L)
Solutions may be loosely categorised as hypertonic, hypotonic or isotonic according to their relative osmolarity
Solutions with a relatively higher osmolarity are categorised as hypertonic (high solute concentration ⇒ gains water)
Solutions with a relatively lower osmolarity are categorised as hypotonic (low solute concentration ⇒ loses water)
Solutions that have the same osmolarity are categorised as isotonic (same solute concentration ⇒ no net water flow)
The osmolarity of a tissue may be interpolated by bathing the sample in solutions with known osmolarities
The tissue will lose water when placed in hypertonic solutions and gain water when placed in hypotonic solutions
Water loss or gain may be determined by weighing the sample before and after bathing in solution
Tissue osmolarity may be inferred by identifying the concentration of solution at which there is no weight change (i.e. isotonic)
Solutions may be loosely categorised as hypertonic, hypotonic or isotonic according to their relative osmolarity
Solutions with a relatively higher osmolarity are categorised as hypertonic (high solute concentration ⇒ gains water)
Solutions with a relatively lower osmolarity are categorised as hypotonic (low solute concentration ⇒ loses water)
Solutions that have the same osmolarity are categorised as isotonic (same solute concentration ⇒ no net water flow)
The osmolarity of a tissue may be interpolated by bathing the sample in solutions with known osmolarities
The tissue will lose water when placed in hypertonic solutions and gain water when placed in hypotonic solutions
Water loss or gain may be determined by weighing the sample before and after bathing in solution
Tissue osmolarity may be inferred by identifying the concentration of solution at which there is no weight change (i.e. isotonic)
Key Terms:
facilitated diffusion
hyper-osmotic phosphorylation cis- pinocytosis hypertonic solution vesicles |
partially permeable
hypo-osmotic intracellular trans- ATP solution |
osmolarity
iso-osmotic extracellular phagocytosis protein pump hypotonic solution solubility |
transport
volume ratio diffusion osmosis isotonic solution |
passive transport
active transport vesicles endocytosis exocytosis equilibrium |
Class Materials:
Osmosis Lab ppt
Osmolarity in the real world ppt
Potato Tissue Practical
Topic 1.4 Review Notes
Topic 1.4 Kahoot Review Quiz
Osmosis Lab ppt
Osmolarity in the real world ppt
Potato Tissue Practical
Topic 1.4 Review Notes
Topic 1.4 Kahoot Review Quiz
PowerPoint presentation Topic 1.4 Cellular Membrane by Chris Paine
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Correct use of terminology is a key skill in Biology. It is essential to use key terms correctly when communicating your understanding, particularly in assessments. Use the quizlet flashcards or other tools such as learn, scatter, space race, speller and test to help you master the vocabulary.
Helpful Links:
Optional Online Practice Quizzes
3 levels of quizzes (Note- not all questions are related to content we are studying.)
Diffusion and Osmosis
How diffusion works, from McGraw Hill
Diffusion applet from Ohio Biosci
Diffusion animation, by John Kyrk
How osmosis works, from McGraw Hill
Clear osmosis animation, from StOlaf.edu
Membrane transport, from Wiley Interactive Concepts in Biochemistry
Isotonic Equilibrium: Osmosis Simulation, from Carnegie Mellon
Facilitated Diffusion
PhET Lab simulation: Membrane Channels (allow Java to run)
Membrane transport, from Wiley Interactive Concepts in Biochemistry
How facilitated diffusion works, from McGraw Hill
Facilitated diffusion, from Northland
Passive transport: simple and facilitated diffusion from Freeman Lifewire
Active Transport & ATP
Membrane transport, from Wiley Interactive Concepts in Biochemistry
Sodium-potassium pump, from McGraw Hill
Sodium-potassium pump, from StOlaf
Active transport from Northland
Active transport from Freeman Lifewire
Vesicle Transport: Endo- and Exocytosis
Vesicle transport from Learn.Genetics
Exocytosis, from StOlaf
Animated plasma membrane, by John Kyrk
Phagocytosis (endocytosis), from McGraw Hill
Endoplasmic reticulum and Golgi apparatus, from Freeman Lifewire
Lysosomes, from McGraw Hill
Membrane Fluidity
Membrane fluidity from Learn.Genetics
Animated plasma membrane, by John Kyrk
Membrane fluidity, from Carnegie Mellon
Membrane fluidity from John Gianni
News:
Cellular Shipping BBC News Health
‘Bouncy’ cell membranes behave like cornstarch and water, from ScienceDaily
Membrane fusion discovered in 2002, from Brookhaven National Laboratory
Video Clips:
Hank Green discusses how molecules come in infinite varieties, so in order to help the complicated chemical world make a little more sense, we classify and categorize them. One of the most important of those classifications is whether a molecule is polar or non-polar, which describes a kind of symmetry - not just of the molecule, but of the charge
Explore the types of passive and active cell transport with the Amoeba Sisters! Transport types covered include simple diffusion, facilitated diffusion, endocytosis, and exocytosis.
Hank describes how cells regulate their contents and communicate with one another via mechanisms within the cell membrane.
Paul Andersen describes how cells move materials across the cell membrane. All movement can be classified as passive or active. Passive transport, like diffusion, requires no energy as particles move along their gradient. Active transport requires additional energy as particles move against their gradient. Specific examples, like GLUT and the Na/K pump are included.
Plasmolysis in Elodea plant
Amoebae are single-celled eukaryotes, which can carry out all the functions of life. The fluidity of their plasma membrane allows them to feed through phagocytosis (endocytosis of solids):
The sodium potassium pump, is responsible for maintaining the electrical charge inside the cell and keep the cells resting potential by pumping out sodium ions and pumping in potassium ions with the help of adenosine triphosphate also known as ATP. Leaky ion channels are also responsible for altering and stabilizing the electrical charge inside the cell and keep the cells resting potentia